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71.
72.
Halichondria panicea (Pallas) is a marine sponge, abundantly occurring in the Adriatic Sea, North Sea, and Baltic Sea. It was the aim of the present
study to investigate if this sponge species harbors bacteria. Cross sections through H. panicea were taken and inspected by electron microscopy. The micrographs showed that this sponge species is colonized by bacteria
in its mesohyl compartment. To identify the bacteria, polymerase chain reaction (PCR) analysis of the 16S rRNA gene segment,
typical for bacteria, was performed. DNA was isolated from sponge material that had been collected near Rovinj (Adriatic Sea),
Helgoland (North Sea), and Kiel (Baltic Sea) and was amplified with bacterial primers by PCR. The data gathered indicate that
in all samples bacteria belonging to the genus Rhodobacter (Proteobacteria, subdivision α) are dominant, suggesting that these bacteria live in symbiotic relationship with the sponge.
In addition, the results show that the different samples taken contain further bacterial species, some of them belonging to
the same genus even though found in sponges from different locations. The possibility of the presence of toxic bacteria was
supported by the finding that organic extracts prepared from sponge samples displayed toxicity, when analyzed in vitro using
leukemia cells.
Received: 7 March 1997 / Accepted: 2 October 1997 相似文献
73.
Kim S. Jacobsen Amy J. Dickman David W. Macdonald Susana Mourato Paul Johnson Lovemore Sibanda Andrew Loveridge 《Conservation biology》2021,35(4):1233-1244
Conflict with humans is one of the major threats facing the world's remaining large carnivore populations, and understanding human attitudes is key to improving coexistence. We surveyed people living near Hwange National Park about their attitudes toward coexisting with lions. We used ordinal regression models with the results of the survey to investigate the importance of a range of tangible and intangible factors on attitudes. The variables investigated included the costs and benefits of wildlife presence, emotion, culture, religion, vulnerability, risk perception, notions of responsibility, and personal value orientations. This was for the purpose of effectively tailoring conservation efforts but also for ethical policy making. Intangible factors (e.g., fear and ecocentric values) were as important as, if not more important than, tangible factors (such as livestock losses) for understanding attitudes, based on the effect sizes of these variables. The degree to which participants’ fear of lions interfered with their daily activities was the most influential variable. The degree to which benefits accrue to households from the nearby protected area was also highly influential, as was number of livestock lost, number of dependents, ecocentric value orientation, and participation in conflict mitigation programs. Contrary to what is often assumed, metrics of livestock loss did not dominate attitudes to coexistence with lions. Furthermore, we found that socioeconomic variables may appear important when studied in isolation, but their effect may disappear when controlling for variables related to beliefs, perceptions, and past experiences. This raises questions about the widespread reliance on socioeconomic variables in the field of human–wildlife conflict and coexistence. To facilitate coexistence with large carnivores, we recommend measures that reduce fear (through education and through protective measures that reduce the need to be fearful), reduction of livestock losses, and ensuring local communities benefit from conservation. Ecocentric values also emerged as influential, highlighting the need to develop conservation initiatives tailored to local values. 相似文献
74.
Arsenic, chromium and mercury removal using mussel shell ash or a sludge/ashes waste mixture 总被引:1,自引:1,他引:0
Natalia Seco-Reigosa Susana Peña-Rodríguez Juan Carlos Nóvoa-Muñoz Manuel Arias-Estévez María J. Fernández-Sanjurjo Esperanza Álvarez-Rodríguez Avelino Núñez-Delgado 《Environmental science and pollution research international》2013,20(4):2670-2678
Different batches of valued mussel shell and waste mussel shell ash are characterised. Shell ash has pH?>?12 and high electrical conductivities (between 16.01 and 27.27 dS?m?1), while calcined shell shows pH values up to 10.7 and electrical conductivities between 1.19 and 3.55 dS?m?1. X-ray fluorescence, nitric acid digestion and water extractions show higher concentrations in shell ash for most parameters. Calcite is the dominant crystalline compound in this ash (95.6 %), followed by aragonite. Adsorption/desorption trials were performed for mussel shell ash and for a waste mixture including shell ash, sewage sludge and wood ash, showing the following percentage adsorptions: Hg(II) >94 %, As(V) >96 % and Cr(VI) between 11 and 30 % for shell ash; Hg(II) >98 %, As(V) >88 % and Cr(VI) between 30 and 88 % for the waste mixture. Hg and As desorption was <5 % for both shell ash and the waste mixture, while Cr desorption was between 92 and 45 % for shell ash, and between 19 and 0 % for the mixture. In view of that, mussel shell ash and the mixture including shell ash, sewage sludge and wood ash could be useful for Hg(II) and As(V) removal. 相似文献
75.
Louise Gallagher Susana Ferreira Frank Convery 《Journal of Environmental Planning and Management》2008,51(2):233-257
One of the most controversial planning issues internationally is the siting of waste disposal infrastructure in local communities. Compensation is viewed as a possible solution to siting difficulties in many countries. However, existing empirical evidence is conflicting as to whether or not compensation-based siting has reduced opposition to such developments. Thus, before compensation policy can be considered as the solution for recognising social costs and introducing equity into the waste planning system, it is important to understand why people reject waste disposal infrastructure developments and if this rejection continues over the lifetime of facility operations. This paper utilises information gathered through surveys to examine the effects of distance, local authority consultation efforts, experience and other factors, on attitudes towards non-hazardous solid waste landfill developments in two examples of a potential and actual host communities. Our findings suggest distance proxies expectations of environmental risk in communities with no experience of living with landfill infrastructure. Community consultations by authorities are consistently important, even after a landfill has been in operation for a number of years. This suggests to policy makers to consult thoroughly and adequately before pursuing compensation policies. 相似文献
76.
Apoptosis in marine sponges: a biomarker for environmental stress (cadmium and bacteria) 总被引:7,自引:0,他引:7
C. Wagner R. Steffen C. Koziol R. Batel M. Lacorn H. Steinhart T. Simat W. E. G. Müller 《Marine Biology》1998,131(3):411-421
The marine demosponge Suberites domuncula is abundantly present on muddy sand bottoms, both in the open sea and in harbors. In the present study it is shown that exposure
of S. domuncula to cadmium (CdCl2) in concentrations ranging from 0.01 to 5.0 g ml−1 for up to 5 d results in apoptotic fragmentation of DNA. Kinetics experiments revealed that after 24 h a significant increase
of DNA fragmentation already occurred. Besides cadmium a second stimulus was identified to also cause apoptosis in this species,
namely exposure to heat-treated Escherichia coli. In order to support the finding that both cadmium and E. coli induce apoptosis in the sponge, expression of the apoptotic gene MA-3 was studied. The cDNA, SDMA3, was isolated and found to be 2247 nucleotides long. The deduced amino acid sequence (Mr 50 765) shares high similarity with the corresponding mouse molecule. Like the mouse gene, the sponge MA-3 gene undergoes increased expression in response to apoptotic stimuli. While the specimens remained alive after treatment
with cadmium, the sponges treated with E. coli died after approximately 12 d. The E. coli-treated animals started to form gemmules 10 to 12 d after addition of the bacteria. Hence, the process of apoptosis in sponges
is triggered by two different pathways, one which is initiated by exogenous factors, e.g. heavy metals, and a second one,
caused by endogenous factors, which leads to gemmule formation and a shift of the presumably immortal cells to mortal cells.
The latter assumption is supported by the finding that during the process of bacteria-induced apoptosis, which results in
the death of the specimens, the activity of the telomerase drops. It is concluded that the cells which appear to be immortal
and telomerase-positive undergo apoptosis during the process of gemmule formation. In consequence cells not involved in the
production of gemmules become mortal. Based on these data, it is proposed that apoptosis is a suitable biomarker in the bioindicator
organism S. domuncula to monitor unfavorable environmental conditions, at least in this animal phylum.
Received: 10 November 1997 / Accepted: 6 March 1998 相似文献
77.
Ana Isabel Barrado Susana García Marisa Luisa Sevillano Jose Antonio Rodríguez Enrique Barrado 《Chemosphere》2013
Thirteen PAHs, five nitro-PAHs and two hydroxy-PAHs were determined in 55 vapor-phase samples collected in a suburban area of a large city (Madrid, Spain), from January 2008 to February 2009. The data obtained revealed correlations between the concentrations of these compounds and a series of meteorological factors (e.g., temperature, atmospheric pressure) and physical–chemical factors (e.g., nitrogen and sulfur oxides). As a consequence, seasonal trends were observed in the atmospheric pollutants. A “mean sample” for the 14-month period would contain a total PAH concentration of 13 835 ± 1625 pg m−3 and 122 ± 17 pg m−3 of nitro-PAHs. When the data were stratified by season, it emerged that a representative sample of the coldest months would contain 18 900 ± 2140 pg m−3 of PAHs and 150 ± 97 pg m−3 of nitro-PAHs, while in an average sample collected in the warmest months, these values drop to 9293 ± 1178 pg m−3 for the PAHs and to 97 ± 13 pg m−3 for the nitro-PAHs. Total vapor phase concentrations of PAHs were one order of magnitude higher than concentrations detected in atmospheric aerosol samples collected on the same dates. Total nitro-PAH concentrations were comparable to their aerosol concentrations whereas vapor phase OH-PAHs were below their limits of the detection, indicating these were trapped in airborne particles. 相似文献
78.
International retirement migration in the Alicante region,Spain: process,spatial pattern and environmental impacts 总被引:1,自引:0,他引:1
Ingo Zasada Susana Alves Felix Claus Müller Annette Piorr Regine Berges Simon Bell 《Journal of Environmental Planning and Management》2010,53(1):125-141
International retirement migration has become an increasing phenomenon in the Mediterranean region of Alicante, Spain. Through an interdisciplinary approach, relationships between the migration process, landscape change and environmental impacts are examined. Lifestyle-related perceptions and demands of UK retirees are studied as a major driving force of urbanisation. The extent and quality of the process is assessed by statistical and land use data analysis. Results reveal a distinctive spatial pattern of in-migration and the emergence of an archetypical landscape, consisting of a mosaic of remaining natural and urbanised landscape along the Mediterranean coast. The paper discusses the necessity of retirees' integration into sustainable planning and decision making. 相似文献
79.
Enzymatic hydrolyzates of recycled paper sludge were tested as suitable feedstock for biological desulfurization by Gordonia alkanivorans strain 1B. Only the hydrolyzate obtained after enzymatic mixture dialysis (dialyzed hydrolyzate) allowed dibenzothiophene (DBT) desulfurization, in spite of faster bacterial growth did occur on non-dialyzed hydrolyzate. For dialyzed hydrolyzate, 250microM DBT was consumed after 96h displaying a maximum specific productivity of 2-hydroxybiphenyl of 1.1micromol g(-1)(dry cell weight) h(-1). A comparison of the kinetics of biodesulfurization was assessed according to the type of hydrolyzate supplementation. Complete consumption of DBT was observed upon the addition of only phosphates and ammonia although further addition of zinc did increase the 2-hydroxybiphenyl production by 14%. Strain 1B was able to desulfurize a model oil containing DBT, 4-methylDBT and 4,6-dimethylDBT, reducing by 63% the total sulfur content in 168h. 相似文献
80.
Abel L. G. Ferreira Pedro Serra Amadeu M. V. M. Soares Susana Loureiro 《Environmental science and pollution research international》2010,17(6):1217-1229
Global warming has become a source of awareness regarding the potential deleterious effects of extreme abiotic factors (e.g.,
temperature, dissolved oxygen (DO) levels) and also their influence on chemicals toxicity. In this work, we studied the combined
effects of nickel and temperature (low and high levels) and nickel and low levels of DO to Daphnia magna, and concentration addition and independent action concepts as well as their deviations for synergism/antagonism, dose ratio
and dose level dependency, were applied to survival and feeding rate data. Nickel single exposure showed an LC50 value for 48 h of 7.36 mg l−1 and an EC50 value for feeding impairment at 2.41 mg l−1. In the acute exposures to high and low temperatures, 50% of mortality was observed, respectively, at 30.7°C and 4.2°C whereas
50% reduction on the feeding activity was recorded at 22.6°C and 16.0°C. Relatively to low DO levels, a LC50 value for 48 h of 0.5 mg l−1 was obtained; feeding activity EC50 value was 2 mg l−1. On acute combined experiments, antagonism was observed for the combination of nickel and extreme temperatures, whereas a
synergistic behaviour was observed in the combined exposure of nickel and low DO levels. At sublethal levels, nickel showed
to be the main inducer of toxicity at high and low temperatures but not at low levels of dissolved oxygen. Toxicokinetics
and toxicodynamics modelling studies should be made in the future to understand the toxicological pathways involved on complex
combinations of stressors and to validate any conclusions. 相似文献